Recombinant Human Coactosin-Like 1 (Dictyostelium), His-tagged

• Cat.No.: COTL1-3377H
• Product Overview: Recombinant human COTL1 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 18.1 kDa (162aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: COTL1 is one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. COTL1 Binds to F-actin in a calcium-independent manner and has no direct effect on actin depolymerization.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT, 0.1M NaCl.
• Purity: > 90% by SDS - PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MATKIDKEAC RAAYNLVRDD GSAVIWVTFK YDGSTIVPGE QGAEYQHFIQ QCTDDVRLFA FVRFTTGDAM SKRSKFALIT WIGENVSGLQ RAKTGTDKTL VKEVVQNFAK EFVISDRKEL EEDFIKSELK KAGGANYDAQ TE.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: COTL1 coactosin-like 1 (Dictyostelium) [ Homo sapiens ]
• Synonyms: CLP; FLJ43657; MGC19733; coactosin-like 1; coactosin-like 1 (Dictyostelium)
• Gene ID: 23406
• mRNA Refseq: NM_021149
• Protein Refseq: NP_066972
• MIM: 606748
• UniProt ID: Q14019
• Chromosome Location: 16q24.1
• Function: actin binding; protein binding