Recombinant Human COASY, GST-tagged

• Cat.No.: COASY-11415H
• Product Overview: Recombinant Human COASY protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: C-term-269a.a.
• Description: Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: COASY CoA synthase [ Homo sapiens ]
• Official Symbol: COASY
• Synonyms: COASY; CoA synthase; Coenzyme A synthase; bifunctional coenzyme A synthase; CoASY; DPCK; NBP; PPAT; nucleotide binding protein; phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase; bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase; UKR1; pOV-2; FLJ35179
• Gene ID: 80347
• mRNA Refseq: NM_001042529
• Protein Refseq: NP_001035994
• MIM: 609855
• UniProt ID: Q13057
• Chromosome Location: 17q12-q21
• Pathway: Coenzyme A biosynthesis, organism-specific biosystem; Coenzyme A biosynthesis, pantothenate => CoA, organism-specific biosystem; Coenzyme A biosynthesis, pantothenate => CoA, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem
• Function: ATP binding; dephospho-CoA kinase activity; nucleotide binding; nucleotidyltransferase activity; pantetheine-phosphate adenylyltransferase activity; transferase activity