Recombinant Human cold shock domain containing C2, RNA binding, His-tagged

• Cat.No.: CSDC2-549H
• Product Overview: CSDC2, 1-153aa, Human His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-153 a.a.
• Description: CSDC2, as known as cold shock domain-containing protein C2, is RNA-binding factor which binds specifically to the very 3"-UTR ends of both histone H1 and H3.3 mRNAs, encompassing the polyadenylation signal. The cold shock domain containing proteins (CSDPs) are one group of the evolutionarily conserved nucleic acid-binding proteins widely distributed in bacteria, plants, animals, and involved in various cellular processes, including adaptation to low temperature, cellular growth, nutrient stress and stationary phase. It may play a central role in the negative regulation of histone variant synthesis in the developing brain. Recombinant human CSDC2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl
• Molecular Mass: 19.2 kDa(176aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTSESTS PPVVPPLHSP KSPVWPTFPF HREGSRVWER GGVPPRDLPS PLPTKRTRTY SATARASAGP VFKGVCKQFS RSQGHGFITP ENGSEDIFVH VSDIEGEYVP VEGDEVTYKM CPIPPKNQKF QAVEVVLTQL APHTPHETWS GQVVGS
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CSDC2 cold shock domain containing C2, RNA binding [ Homo sapiens ]
• Official Symbol: CSDC2
• Synonyms: CSDC2; cold shock domain containing C2, RNA binding; cold shock domain-containing protein C2; PIPPin; RNA-binding protein pippin; PIPPIN; dJ347H13.2
• Gene ID: 27254
• mRNA Refseq: NM_014460
• Protein Refseq: NP_055275
• UniProt ID: Q9Y534
• Chromosome Location: 22q13.2
• Function: DNA binding; RNA binding