Recombinant Human COVID-19 Spike RBD protein

• Cat.No.: S-1453V
• Product Overview: Recombinant Human COVID-19 Spike RBD protein was expressed in E. coli.

Specification

• Species: COVID-19
• Source: E.coli
• Tag: Non
• Form: Lyophilized from a 0.2 µm filtered concentrated solution in 30% acetonitrile and 0.1% TFA.
• Molecular Mass: Approximately 25.1 kDa, a single non-glycosylated polypeptide chain containing 223 amino acid residues.
• AASequence: RVQPTESIVR FPNITNLCPF GEVFNATRFA SVYAWNRKRI SNCVADYSVL YNSASFSTFK CYGVSPTKLN DLCFTNVYAD SFVIRGDEVR QIAPGQTGKI ADYNYKLPDD FTGCVIAWNS NNLDSKVGGN YNYLYRLFRK SNLKPFERDI STEIYQAGST PCNGVEGFNC YFPLQSYGFQ PTNGVGYQPY RVVVLSFELL HAPATVCGPK KSTNLVKNKC VNF
• Endotoxin: Less than 0.1 EU/μg of rSARS-CoV-2 Spike RBD as determined by LAL method.
• Purity: > 90% by SDS-PAGE.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.