Recombinant Human coxsackievirus B1 VP1 Antigen, His tagged

• Cat.No.: VP1-077H
• Product Overview: The polyprotein from coxsackievirus B1 (Tucson) (CVB1) has been prepared as a recombinant antigen fused to a his-tag. It is produced from the immune dominant regions of the capsid protein.

Specification

• Species: Human coxsackievirus B1
• Source: E.coli
• Tag: His
• Description: VP1 contains serotype specific neutralization sites and hence it is a key genotyping determinant. The structural polyprotein of the virus contains the proteins VP1 to VP4. VP1, VP2 and VP3 are the most immunogenic proteins.
• Tag: N-His
• Molecular Mass: Determined by SDS-PAGE, the protein band is between molecular markers of 25-35 kDa, while relative molecular mass calculated from amino acid sequence is 26,665.6 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 8, 0.15 M NaCl, 0.1% polyoxyethylene (10) tridecyl ether and 8 M urea
• Concentration: 1.45 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Ray, C. G., L. L. Minnich, and P. C. Johnson. Selective polymyositis induced by coxsackievirus B1 in mice. 1979, J. Infect. Dis. 140:239-243.
  2. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.