Recombinant Human CPA4 protein, His-tagged

• Cat.No.: CPA4-7970H
• Product Overview: Recombinant Human CPA4 protein (Gly17-Tyr421), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Description: Carboxypeptidases are zinc-containing exopeptidases that catalyze the release of carboxy-terminal amino acids, and are synthesized as zymogens that are activated by proteolytic cleavage. Carboxypeptidases cleave amino acids from the C-terminus of proteins and peptides and many are metalloproteases. Carboxypeptidase A4 (CPA4) is also known as Carboxypeptidase A3 (CPA3), which belongs to the peptidase M14 family. Fetal express the protein in the adrenal gland, brain, heart, intestine, kidney, liver and lung.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 46.4 kDa. The protein migrates as 48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: 405
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CPA4
• Official Symbol: CPA4
• Synonyms: CPA4; carboxypeptidase A4; carboxypeptidase A3; CPA3
• Gene ID: 51200
• mRNA Refseq: NM_001163446
• Protein Refseq: NP_001156918
• MIM: 607635
• UniProt ID: Q9UI42