Recombinant Human CPSF1 protein, His & T7-tagged

• Cat.No.: CPSF1-1667H
• Product Overview: Recombinant Human CPSF1 aa. (Met1~Leu300 (Accession # Q10570)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis. CPSF1 is the largest subunit of the CPSF complex (Murthy and Manley, 1995 [PubMed 7590244]).
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 37.3kDa
• Protein length: Met1~Leu300 (Accession # Q10570)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 6.1

Gene Information

• Gene Name: CPSF1 cleavage and polyadenylation specific factor 1 [ Homo sapiens (human) ]
• Official Symbol: CPSF1
• Synonyms: CPSF1; cleavage and polyadenylation specific factor 1; CPSF160; P/cl.18; HSU37012; cleavage and polyadenylation specificity factor subunit 1; CPSF 160 kDa subunit; cleavage and polyadenylation specific factor 1, 160kDa; cleavage and polyadenylation specificity factor 160 kDa subunit; polyadenylation specificity factor
• Gene ID: 29894
• mRNA Refseq: NM_013291.2
• Protein Refseq: NP_037423.2
• UniProt ID: Q10570