Recombinant Human Creatine Kinase MB Isoenzyme Type-Ii

• Cat.No.: CKMBITII-25H
• Product Overview: Recombinant Human CKMBITII produced in Pichia Pastoris is a glycosylated polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to MB Isoenzyme in ELISA. The CKMBITII is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: P.pastoris
• Tag: Non
• Description: CK-MB Type II possesses the naturally occurring carboxy-terminal amino acid lysine. This occurs during a myocardial infarct (MI or heart attack) when CK-MB Type II is released from damaged heart muscle, and the C-terminal lysine is cleaved in the blood stream, thus creating CK-MB Type I. This difference can be exploited in diagnosis of an MI.
• Physical Appearance: Sterile Filtered colourless liquid formulation.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: Each mg of protein (15.8mg/ml) contains 20mM Tris-HCl, pH-6.8, 1mM EDTA and 1mM DTT.
• Biological Activity: The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 µmole creatine phosphate was 892 IU/mg at 37 degrees celsius.
• Storage: CKMBITII although stable at 15℃ for 7 days, should be stored desiccated below -18℃. Please prevent freeze-thaw cycles.

Gene Information

• Synonyms: Creatine Kinase MB Isoenzyme Type-II, CKMBITII, CKMBI, CKMB.