Recombinant Human Crystallin, Beta B1, His-tagged

• Cat.No.: CRYBB1-2495H
• Product Overview: Recombinant human CRYBB1 protein, fused to His-tag at C-terminus was expressed inE.coliand purified by using conventional chromatography techniques.MW = 29.1 kDa (260aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Crystallins are the major structural proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into α, β, and γ families. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. CRYBB1 is a beta basic group member and undergoes extensive cleavage at its N-terminal extension during lens maturation.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 1mM DTT.
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MSQAAKASAS ATVAVNPGPD TKGKGAPPAG TSPSPGTTLA PTTVPITSAK AAELPPGNYR LVVFELENFQ GRRAEFSGEC SNLADRGFDR VRSIIVSAGP WVAFEQSNFR GEMFILEKGE YPRWNTWSSS YRSDRLMSFR PIKMDAQEHK ISLFEGANFK GNTIEIQGDD APSLWVYGFS DRVGSVKVSS GTWVGYQYPG YRGYQYLLEP GDFRHWNEWG AFQPQMQSLR RLRDKQWHLE GSFPVLATEP PKRSHHHHHH.
• Storage: can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CRYBB1 crystallin, beta B1 [ Homo sapiens ]
• Synonyms: crystallin, beta B1; CATCN3; CRYBB1; beta-crystallin B1; beta-B1 crystallin; eye lens structural protein; Beta-B1 crystallin
• Gene ID: 1414
• mRNA Refseq: NM_001887
• Protein Refseq: NP_001878
• MIM: 600929
• UniProt ID: P53674
• Chromosome Location: 22q11.2; 22q12.1
• Function: structural constituent of eye lens