Active Recombinant Human CSF3R protein, His-tagged

• Cat.No.: CSF3R-8526H
• Product Overview: Active Recombinant Human CSF3R protein(Met 1-Pro 621)(NP_000751.1) precursor was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-621 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to inhibit the G-CSF induced proliferation of NFS60 mouse myeloid cells. The ED50 for this effect is typically 20-80 ng/mL.
• Molecular Mass: The mature recombinant human GCSFR consists of 608 amino acids and predicts a molecular mass of 68 kDa by SDS-PAGE under reducing conditions, the apparent molecular mass of rhGCSFR is approximately 92 kDa due to glycosylation.
• Endotoxin: < 1 EU/µg as determined by LAL test.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CSF3R colony stimulating factor 3 receptor (granulocyte) [ Homo sapiens ]
• Official Symbol: CSF3R
• Synonyms: CSF3R; colony stimulating factor 3 receptor (granulocyte); CD114; granulocyte colony-stimulating factor receptor; GCSFR; G-CSF-R; CD114 antigen; G-CSF receptor
• Gene ID: 1441
• mRNA Refseq: NM_000760
• Protein Refseq: NP_000751
• MIM: 138971
• UniProt ID: Q99062