Active Recombinant Human CSNK1E Protein, GST-tagged

• Cat.No.: CSNK1E-1994H
• Product Overview: Human CSNK1E (NP_001885.1, 1 a.a. - 348 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.

Specification

• Description: The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]
• Source: Insect Cells
• Species: Human
• Tag: GST
• Form: Liquid
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CKtide. ATP: 100 uM.
• Molecular Mass: 68 kDa
• Purity: 54 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: CSNK1E casein kinase 1, epsilon [ Homo sapiens ]
• Official Symbol: CSNK1E
• Synonyms: CSNK1E; casein kinase 1, epsilon; casein kinase I isoform epsilon; CKIE; CKIepsilon; HCKIE; CKIe; CKI-epsilon; MGC10398
• Gene ID: 1454
• mRNA Refseq: NM_001894
• Protein Refseq: NP_001885
• MIM: 600863
• UniProt ID: P49674