Recombinant Human CSNK1E Protein, GST-tagged


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Recombinant Human CSNK1E Protein, GST-tagged

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Cat.No. : CSNK1E-1994H
Product Overview : Human CSNK1E (NP_001885.1, 1 a.a. - 348 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Description : The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]
Source : Insect Cells
Species : Human
Tag : GST
Form : Liquid
Bio-activity : The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CKtide. ATP: 100 uM.
Molecular Mass : 68 kDa
Purity : 54 % by SDS-PAGE/CBB staining
Applications : Functional Study
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Quality Control Testing: :

Loading 1 μg protein in SDS-PAGE

Result of activity analysis :

Gene Name : CSNK1E casein kinase 1, epsilon [ Homo sapiens ]
Official Symbol : CSNK1E
Synonyms : CSNK1E; casein kinase 1, epsilon; casein kinase I isoform epsilon; CKIE; CKIepsilon; HCKIE; CKIe; CKI-epsilon; MGC10398;
Gene ID : 1454
mRNA Refseq : NM_001894
Protein Refseq : NP_001885
MIM : 600863
UniProt ID : P49674

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