Recombinant Human CST1 protein, His-tagged
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|Product Overview :||Recombinant Human CST1 (Trp21-Ser141) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).|
|Description :||Cystatin-1 (CST1) is also known as Cystatin-SN, Cystain-SA-I, Salivary cystatin-SA-1, which is secreted protein and belongs to the cystatin family. Cystatin-1 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level) and also expressed in saliva, tears, urine and seminal fluid. Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well.|
|Predicted N Terminal :||Trp21|
|Form :||Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH6.0, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 15.1 kDa. The protein migrates as 17-19 kDa under reducing (R) condition (SDS-PAGE).|
|Protein length :||Trp21-Ser141|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|Gene Name :||CST1|
|Official Symbol :||CST1|
|Synonyms :||CST1; cystatin SN; cystatin-SN; cystatin 1; cystatin-1; cystain-SA-I; cystatin SA-I; salivary cystatin-SA-1; cysteine proteinase inhibitor, type 2 family|
|Gene ID :||1469|
|mRNA Refseq :||NM_001898|
|Protein Refseq :||NP_001889|
|UniProt ID :||P01037|
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