Active Recombinant Human CST7 protein, His-tagged

• Cat.No.: CST7-3149H
• Product Overview: Active Recombinant Human CST7 protein(NP_003641.3) (Met 1-His 145) was expressed in HEK293，with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-145 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC. The IC50 value is <6 nM.
• Molecular Mass: Cystatin F exists as a disulfide-linked homodimer. The human cystatin F monomer consists of 137 amino acids and and has a predicted molecular mass of 16.1 kDa. As a result of glycosylation, the recombinant Cystatin F migrates as an approximately 44 kDa band in SDS-PAGE under non-reduced conditions corresponding to the dimmer.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CST7 cystatin F (leukocystatin) [ Homo sapiens ]
• Official Symbol: CST7
• Synonyms: CST7; cystatin F (leukocystatin); cystatin-F; cystatin-7; leukocystatin; cystatin-like metastasis-associated protein; CMAP
• Gene ID: 8530
• mRNA Refseq: NM_003650
• Protein Refseq: NP_003641
• MIM: 603253
• UniProt ID: O76096