|Product Overview :||Recombinant Human CTGF protein (Gln27-Ala349), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).|
|Description :||Connective Tissue Growth Factor (CTGF), also known as CCN2, is a member of the CCN (CCN1-6) family of modular matricellular proteins. Like other CCN proteins, mature human CTGF consists of IGF-binding protein domain, a vWF-C domain, a TSP-1 domain, and a cysteine knot heparin-binding domain. CTGF promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Analysis of CCN2 function in vivo has focused primarily on its key role as a mediator of excess ECM synthesis in multiple fibrotic diseases.|
|Form :||Lyophilized from 0.22 um filtered solution in Tris with Potassium glutamate and Arginine, pH7.0, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 37.4 kDa. The protein migrates as 38-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||323|
|Endotoxin :||Less than 1.0 EU per ug by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of CTGF-392H :||
Human CTGF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Bioactivity-ELISA of CTGF-392H :||
Immobilized Human CTGF, His Tag at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CTGF Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).
|Gene Name :||CTGF|
|Official Symbol :||CTGF|
|Synonyms :||CTGF; connective tissue growth factor; CCN2; IGFBP8; IBP-8; IGFBP-8; CCN family member 2; IGF-binding protein 8; hypertrophic chondrocyte-specific protein 24; insulin-like growth factor-binding protein 8; NOV2; HCS24; MGC102839|
|Gene ID :||1490|
|mRNA Refseq :||NM_001901|
|Protein Refseq :||NP_001892|
|UniProt ID :||P29279|