Recombinant Human CYP27B1 Protein, GST-tagged

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Recombinant Human CYP27B1 Protein, GST-tagged

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Cat.No. : CYP27B1-2254H
Product Overview : Human CYP27B1 full-length ORF (BAG37458.1, 1 a.a. - 508 a.a.) recombinant protein with GST-tag at N-terminal.
Description : This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I. [provided by RefSeq, Jul 2008]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 82.28 kDa
AA Sequence : MTQTLKYASR VFHRVRWAPE LGASLGYREY HSARRSLADI PGPSTPSFLA ELFCKGGLSR LHELQVQGAA HFGPVWLASF GTVRTVYVAA PALVEELLRQ EGPRPERCSF SPWTEHRRCR QRACGLLTAE GEEWQRLRSL LAPLLLRPQA AARYAGTLNN VVCDLVRRLR RQRGRGTGPP ALVRDVAGEF YKFGLEGIAA VLLGSRLGCL EAQVPPDTET FIRAVGSVFV STLLTMAMPH WLRHLVPGPW GRLCRDWDQM FAFAQRHVER REAEAAMRNG GQPEKDLESG AHLTHFLFRE ELPAQSILGN VTELLLAGVN TVSNTLSWAL YELSRHPEVQ TALHSEITAA LSPGSSAYPS ATVLSQLPLL KAVVKEVLRL YPVVPGNSRV PDKDIHVGDY IIPKNTLVTL CHYATSRDPA QFPEPNSFRP ARWLGEGPTP HPFASLPFGF GKRSCMGRRL AELELQMALA QILTHFEVQP EPGAAPVRPK TRTVLVPERS INLQFLDR
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Gene Name : CYP27B1 cytochrome P450, family 27, subfamily B, polypeptide 1 [ Homo sapiens ]
Official Symbol : CYP27B1
Synonyms : CYP27B1; cytochrome P450, family 27, subfamily B, polypeptide 1; cytochrome P450, subfamily XXVIIB (25 hydroxyvitamin D 1 alpha hydroxylase), polypeptide 1 , PDDR, VDD1; 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial; 1alpha(OH)ase; 25 Hydroxyvitamin D3 1alpha hydroxylase; CYP1; P450c1; VDDR I; VD3 1A hydroxylase; cytochrome p450 27B1; cytochrome P450C1 alpha; cytochrome P450VD1-alpha; calcidiol 1-monooxygenase; 25-OHD-1 alpha-hydroxylase; 25 hydroxyvitamin D3-1-alpha hydroxylase; cytochrome P450 subfamily XXVIIB polypeptide 1; VDR; CP2B; PDDR; VDD1; VDDR; VDDRI; CYP27B; CYP1alpha;
Gene ID : 1594
mRNA Refseq : NM_000785
Protein Refseq : NP_000776
MIM : 609506
UniProt ID : O15528

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