Recombinant Human CYP3A4/NADPH reductase protein
Cat.No. : | CYP3A4-33H |
Product Overview : | This is a permeabilized and stabilized dried yeast powder preparation containing recombinant human CYP 3A4 and recombinant human P450 NADPH oxidoreductase co-expressed in the same preparation. |
- Specification
- Gene Information
- Related Products
Source : | Pichia pastoris |
Species : | Human |
Form : | Lyophilized powder |
Molecular Mass : | 55 Kda |
Characteristic : | - Co-expresses human CYP3A4 and human P450 oxidoreductase (hCPR) allowing easy reconstitution of the endogenous system- Useful for all the applications as a baculosome system but more cost-effective- Easy to handle dry powder- Very stable in dry form and active in multiple buffers and solvents during long incubations- Addition of NADP+ & Glucose-6-Phosphate are not essential (but will boost T3activity)- After the reaction, the protein can be pelleted at relatively low speeds- Clean HPLC profiles for easy metabolite ID and purification- Highly controlled production process for lot-to-lot reproducibility |
Notes : | Centrifuge the vial prior to opening |
Storage : | Stable for 1 year as supplied. Store dry powder at -20 centigrade. Reconstitute in 100 mM Potassium Phosphate buffer, pH 7.7. Not recommended to be stored as solution. If storing as solution, aliquot and store at -80 centigrade. Avoid repeated freeze/thaw cycles and use aliquots within one month (the human CYP3A4 will lose approximately 10% activity per week when stored at -80 centigrade). Thaw aliquots rapidly at 37 centigrade and place on ice until use (thawed aliquots should be used within 4 hours). |
Reconstitution : | 100 mM Potassium Phosphate buffer, pH 7.7 |
Gene Name : | CYP3A4 cytochrome P450, family 3, subfamily A, polypeptide 4 [ Homo sapiens ] |
Official Symbol : | CYP3A4 |
Synonyms : | CYP3A4; cytochrome P450, family 3, subfamily A, polypeptide 4; CYP3A3, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; cytochrome P450 3A4; nifedipine oxidase; cytochrome P450 3A3; cytochrome P450 HLp; cytochrome P450-PCN1; cytochrome P450 NF-25; albendazole sulfoxidase; quinine 3-monooxygenase; albendazole monooxygenase; P450-III, steroid inducible; glucocorticoid-inducible P450; taurochenodeoxycholate 6-alpha-hydroxylase; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1; MGC126680; |
Gene ID : | 1576 |
mRNA Refseq : | NM_001202855 |
Protein Refseq : | NP_001189784 |
MIM : | 124010 |
UniProt ID : | P08684 |
Chromosome Location : | 7q21.1 |
Pathway : | Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Biological oxidations, organism-specific biosystem; Codeine and morphine metabolism, organism-specific biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem; Drug metabolism - cytochrome P450, organism-specific biosystem; Drug metabolism - cytochrome P450, conserved biosystem; |
Function : | albendazole monooxygenase activity; caffeine oxidase activity; electron carrier activity; enzyme binding; heme binding; iron ion binding; metal ion binding; monooxygenase activity; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding; quinine 3-monooxygenase activity; steroid binding; steroid hydroxylase activity; taurochenodeoxycholate 6alpha-hydroxylase activity; testosterone 6-beta-hydroxylase activity; vitamin D 24-hydroxylase activity; vitamin D3 25-hydroxylase activity; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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