Recombinant Human Cystatin B (Stefin B), His-tagged

• Cat.No.: CSTB-299H
• Product Overview: Recombinant CSTB, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques (118 aa, 13kDa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: CSTB, also known as Cystatin B is an anti-protease implicated in myoclonus epilepsy, a degenerative disease of the central nervous system. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein is able to form a dimer stabilized by noncovalent forces and is thought to play a role in protecting against the proteases leaking from lysosomes. In cells, CSTB is located in the lysosomes and the cytoplasm, but also in the nucleus.
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 50 mM NaCl.
• Molecular Weight: 13 kDa (118 aa).
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method).
• Sequence: MGSSHHHHHH SSGLVPRGSH MMCGAPSATQ PATAETQHIA DQVRSQLEEK ENKKFPVFKA VSFKSQVVAG TNYFIKVHVG DEDFVHLRVF QSLPHENKPL TLSNYQTNKA KHDELTYF
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CSTB
• Synonyms: CSTB; cystatin B (stefin B); PME; CST6; EPM1; STFB; CPI-B; Stefin-B; cystatin B; stefin B; cystatin B (stefin B); Liver thiol proteinase inhibitor; cystatin B (liver thiol proteinase inhibitor).
• Gene ID: 1476
• mRNA Refseq: NM_000100.2
• Protein Refseq: NP_000091.1
• MIM: 601145
• UniProt ID: P04080
• Chromosome Location: 21q22.3
• Function: cysteine-type endopeptidase inhibitor activity; protease binding; protein binding.