Recombinant Human DBH protein, His & T7-tagged

• Cat.No.: DBH-6945H
• Product Overview: Recombinant Human DBH aa. (His335~Val571 (Accession # P09172)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 30.6kDa
• Protein length: His335~Val571 (Accession # P09172)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 7.7.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: DBH dopamine beta-hydroxylase [ Homo sapiens (human) ]
• Official Symbol: DBH
• Synonyms: DBH; dopamine beta-hydroxylase; DBM; dopamine beta-hydroxylase; dopamine beta-hydroxylase (dopamine beta-monooxygenase)
• Gene ID: 1621
• mRNA Refseq: NM_000787.3
• Protein Refseq: NP_000778.3
• UniProt ID: P09172