Recombinant Human DBR1, His-tagged

• Cat.No.: DBR1-26123TH
• Product Overview: Recombinant fragment, corresponding to amino acids 110-554 of Human DBR1 with an N terminal His tag. Predicted mwt: 50 kDa

Specification

• Description: The RNA lariat debranching enzyme, or DBR1, specifically hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules.
• Conjugation: HIS
• Source: E. coli
• Form: Lyophilised:Reconstitute with 90 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: AGVVKYRGVRIGGISGIFKSHDYRKGHFECPPYNSSTIRS IYHVRNIEVYKLKQLKQPIDIFLSHDWPRSIYHYGNKK QLLKTKSFFRQEVENNTLGSPAASELLEHLKPTYWFSAHL HVKFAALMQHQAKDKGQTARATKFLALDKCLPHRDFLQ ILEIEHDPSAPDYLEYDIEWLTILRATDDLINVTGRLW NMPENNGLHARWDYSATEEGMKEVLEKLNHDLKVPCNFSV TAACYDPSKPQTQMQLIHRINPQTTEFCAQLGIIDINV RLQKSKEEHHVCGEYEEQDDVESNDSGEDQSEYNTDTS ALSSINPDEIMLDEEEDEDSIVSAHSGMNTPSVEPSDQAS EFSASFSDVRILPGSMIVSSDDTVDSTIDREGKPGGTV ESGNGEDLTKVPLKRLSDEHEPEQRKKIKRRNQAIYAAVDDDDDDAA

Gene Information

• Gene Name: DBR1 debranching enzyme homolog 1 (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: DBR1
• Synonyms: DBR1; debranching enzyme homolog 1 (S. cerevisiae); debranching enzyme (S. Cerevisiae) homolog 1; lariat debranching enzyme
• Gene ID: 51163
• mRNA Refseq: NM_016216
• Protein Refseq: NP_057300
• MIM: 607024
• Uniprot ID: Q9UK59
• Chromosome Location: 3q22.3
• Function: RNA lariat debranching enzyme activity; hydrolase activity, acting on ester bonds; metal ion binding