"DBT" Related Products


Recombinant Human DBT protein, His-tagged

Cat.No.: DBT-4930H
Product Overview: Recombinant Human DBT fused with hexa-histidine purification tag was expressed in Insect cells.
Description: The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Source: Insect cells
Species: Human
Tag: His
Form: DLST (0.91mg/ml) is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl and 20% glycerol.
Molecular Mass: 47,321 Dalton
Purity: Greater than 75% as determined by SDS-PAGE.
Applications: Western blot with anti-M2-Antigen autoantibody-positive patient sera or monoclonal anti-hexa-His-tag antibody.Coating concentration; 0.4-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels.0.4-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Storage: Store at 4 centigradeif entire vial will be used within 2-4 weeks. Store, frozen at -20 centigradefor longer periods of time.Avoid multiple freeze-thaw cycles.
Gene Name: DBT dihydrolipoamide branched chain transacylase E2 [ Homo sapiens ]
Official Symbol: DBT
Synonyms: DBT; BCKADE2; BCKAD-E2; BCKAD E2 subunit; dihydrolipoyl transacylase; E2; E2B; BCATE2; MGC9061;
Gene ID: 1629
mRNA Refseq: NM_001918
Protein Refseq: NP_001909
MIM: 248610
UniProt ID: P11182
Chromosome Location: 1p31
Pathway: 2-oxobutanoate degradation I, organism-specific biosystem; 2-oxobutanoate degradation I, conserved biosystem; Branched-chain amino acid catabolism, organism-specific biosystem
Function: cofactor binding; dihydrolipoyllysine-residue (2-methylpropanoyl)transferase activity; transferase activity, transferring acyl groups;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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