Recombinant Human dCMP Deaminase, His-tagged

• Cat.No.: DCTD-732H
• Product Overview: Recombinant human DCTD protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 22.1 kDa (198aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: DCTD (deoxycytidylate deaminase), also known as dCMP deaminase, is a 178 amino acid allosteric enzyme that exists as a homohexamer and belongs to the cytidine and deoxycytidylate deaminase protein family. Using zinc as a cofactor, DCTD catalyzes the deamination of dCMP to dUMP, thereby producing the nucleotide substrate (dUMP) that is used by thymidylate synthase.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MSEVSCKKRD DYLEWPEYFM AVAFLSAQRS KDPNSQVGAC IVNSENKIVG IGYNGMPNGC SDDVLPWRRT AENKLDTKYP YVCHAELNAI MNKNSTDVKG CSMYVALFPC NECAKLIIQA GIKEVIFMSD KYHDSDEATA ARLLFNMAGV TFRKFIPKCS KIVIDFDSIN SRPSQKLQ
• Purity: > 90% by SDS – PAGE.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 1mM DTT, 20% glycerol.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: DCTD dCMP deaminase [ Homo sapiens ]
• Synonyms: DCTD; dCMP deaminase; MGC111062; deoxycytidylate deaminase; EC 3.5.4.12
• Gene ID: 1635
• mRNA Refseq: NM_001012732
• Protein Refseq: NP_001012750
• MIM: 607638
• UniProt ID: P32321
• Chromosome Location: 4q35.1
• Pathway: Metabolic pathways; Pyrimidine metabolism
• Function: dCMP deaminase activity; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds; metal ion binding; zinc ion binding