Description : |
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined. |
Source : |
HEK293 |
Species : |
Human |
Tag : |
Myc/DDK |
Molecular Mass : |
95.6 kDa |
AA Sequence : |
MAGELADKKDRDASPSKEERKRSRT PDRERDRDRDRKSSPSKDRKRHRSR DRRRGGSRSRSRSRSKSAERERRHK ERERDKERDRNKKDRDRDKDGHRRD KDRKRSSLSPGRGKDFKSRKDRDSK KDEEDEHGDKKPKAQPLSLEELLAK KKAEEEAEAKPKFLSKAEREAEALK RRQQEVEERQRMLEEERKKRKQFQD LGRKMLEDPQERERRERRERMERET NGNEDEEGRQKIREEKDKSKELHAI KERYLGGIKKRRRTRHLNDRKFVFE WDASEDTSIDYNPLYKERHQVQLLG RGFIAGIDLKQQKREQSRFYGDLME KRRTLEEKEQEEARLRKLRKKEAKQ RWDDRHWSQKKLDEMTDRDWRIFRE DYSITTKGGKIPNPIRSWKDSSLPP HILEVIDKCGYKEPTPIQRQAIPIG LQNRDIIGVAETGSGKTAAFLIPLL VWITTLPKIDRIEESDQGPYAIILA PTRELAQQIEEETIKFGKPLGIRTV AVIGGISREDQGFRLRMGCEIVIAT PGRLIDVLENRYLVLSRCTYVVLDE ADRMIDMGFEPDVQKILEHMPVSNQ KPDTDEAEDPEKMLANFESGKHKYR QTVMFTATMPPAVERLARSYLRRPA VVYIGSAGKPHERVEQKVFLMSESE KRKKLLAILEQGFDPPIIIFVNQKK GCDVLAKSLEKMGYNACTLHGGKGQ EQREFALSNLKAGAKDILVATDVAG RGIDIQDVSMVVNYDMAKNIEDYIH RIGRTGRAGKSGVAITFLTKEDSAV FYELKQAILESPVSSCPPELANHPD AQHKPGTILTKKRREETIFATRTRP LEQKLISEEDLAANDILDYKDDDDK V |
Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : |
50 μg/mL as determined by BCA |
Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |