Recombinant Human DDX4 protein, His-tagged

• Cat.No.: DDX4-182H
• Product Overview: Recombinant Human DDX4 protein(NP_001136021)(340-690 aa), fused with His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.). 5 % trehalose and 5 % mannitol are added as protectants before lyophilization.
• Protein length: 340-690 aa
• AA Sequence: TRELVNKIYLEARKFSFGTCVRAVV IYGGTQLGHSIRQIVQGCNILCATP GRLMDIIGKEKIGLKQIKYLVLDEA DRMLDMGFGPEMKKLISCPGMPSKE QRQTLMFSATFPEEIQRLAAEFLKS NYLFVAVGQVGGACRDVQQTVLQVG QFSKREKLVEILRNIGDERTMVFVE TKKKADFIATFLCQEKISTTSIHGD REQREREQALGDFRFGKCPVLVATS VAARGLDIENVQHVINFDLPSTIDE YVHRIGRTGRCGNTGRAISFFDLES DNHLAQPLVKVLTDAQQDVPAWLEE IAFSTYIPGFSGSTRGNVFASVDTR KGKSTLNTAGFSSSQAPNPVDDESW D
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: DDX4 DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 [ Homo sapiens ]
• Official Symbol: DDX4
• Synonyms: DDX4; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 4; probable ATP-dependent RNA helicase DDX4; VASA; vasa homolog; DEAD box protein 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; MGC111074
• Gene ID: 54514
• mRNA Refseq: NM_001142549
• Protein Refseq: NP_001136021
• MIM: 605281
• UniProt ID: Q9NQI0