| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
32-266 aa |
| Description : |
Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. |
| Form : |
Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4). |
| Bio-activity : |
Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. |
| Molecular Mass : |
26.87 kDa |
| Endotoxin : |
< 1 EU/μg by LAL |
| Purity : |
> 95% as determined by Bis-Tris PAGE |
| Notes : |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use. |
| Storage : |
Upon receiving, the product remains stable for 6 months at -20 centigrade or below. Upon reconstitution, the product should be stable for 3 months at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water. |
