Active Recombinant Human DKK1 protein, Fc-tagged

• Cat.No.: DKK1-313H
• Product Overview: Active Recombinant Human DKK1 protein(O94907)(Thr32-His266) is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 32-266 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human DKK1, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 1.69μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 52.54 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: DKK1 dickkopf 1 homolog (Xenopus laevis) [ Homo sapiens ]
• Official Symbol: DKK1
• Synonyms: DKK1; dickkopf 1 homolog (Xenopus laevis); dickkopf (Xenopus laevis) homolog 1; dickkopf-related protein 1; DKK 1; SK; hDkk-1; dickkopf-1 like; dickkopf related protein-1; DKK-1
• Gene ID: 22943
• mRNA Refseq: NM_012242
• Protein Refseq: NP_036374
• MIM: 605189
• UniProt ID: O94907