Recombinant Human DKKL1 protein, hFc-tagged

• Cat.No.: DKKL1-6920H
• Product Overview: Recombinant Human DKKL1 protein(Q9UK85) (Ala 31-Leu 242) was expressed in HEK293 with the fused Fc region of human IgG1 at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 31-242 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is typically 5-15 μg/ml.
• Molecular Mass: The recombinant human DKKL1/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 472 amino acids and has a predicted molecular mass of 52.4 kDa. rhDKKL1/Fc monomer migrates as an approximately 60 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 70 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DKKL1 dickkopf-like 1 [ Homo sapiens ]
• Official Symbol: DKKL1
• Synonyms: DKKL1; dickkopf-like 1; dickkopf like 1 (soggy); dickkopf-like protein 1; cancer/testis antigen 34; CT34; SGY 1; soggy; soggy-1; protein soggy-1; SGY; SGY1; SGY-1
• Gene ID: 27120
• mRNA Refseq: NM_001197301
• Protein Refseq: NP_001184230
• MIM: 605418
• UniProt ID: Q9UK85