Recombinant Human DLD Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: DLD-6687H
• Product Overview: DLD MS Standard C13 and N15-labeled recombinant protein (NP_000099) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 54.2 kDa
• AA Sequence: MQSWSRVYCSLAKRGHFNRISHGLQ GLSAVPLRTYADQPIDADVTVIGSG PGGYVAAIKAAQLGFKTVCIEKNET LGGTCLNVGCIPSKALLNNSHYYHM AHGKDFASRGIEMSEVRLNLDKMME QKSTAVKALTGGIAHLFKQNKVVHV NGYGKITGKNQVTATKADGGTQVID TKNILIATGSEVTPFPGITIDEDTI VSSTGALSLKKVPEKMVVIGAGVIG VELGSVWQRLGADVTAVEFLGHVGG VGIDMEISKNFQRILQKQGFKFKLN TKVTGATKKSDGKIDVSIEAASGGK AEVITCDVLLVCIGRRPFTKNLGLE ELGIELDPRGRIPVNTRFQTKIPNI YAIGDVVAGPMLAHKAEDEGIICVE GMAGGAVHIDYNCVPSVIYTHPEVA WVGKSEEQLKEEGIEYKVGKFPFAA NSRAKTNADTDGMVKILGQKSTDRV LGAHILGPGAGEMVNEAALALEYGA SCEDIARVCHAHPTLSEAFREANLA ASFGKSINFTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: DLD dihydrolipoamide dehydrogenase [ Homo sapiens (human) ]
• Official Symbol: DLD
• Synonyms: DLD; dihydrolipoamide dehydrogenase; dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2 oxo glutarate complex, branched chain keto acid dehydrogenase complex), GCSL, LAD; dihydrolipoyl dehydrogenase, mitochondrial; DLDH; E3 component of pyruvate dehydrogenase complex; 2 oxo glutarate complex; branched chain keto acid dehydrogenase complex; diaphorase; lipoamide reductase; lipoyl dehydrogenase; lipoamide dehydrogenase; glycine cleavage system L protein; glycine cleavage system protein L; E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex; E3; LAD; GCSL; PHE3
• Gene ID: 1738
• mRNA Refseq: NM_000108
• Protein Refseq: NP_000099
• MIM: 238331
• UniProt ID: P09622