|Product Overview:||Human DLG1 cell lysate, transcript variant 2, was derived from Human HEK293T cell line.|
|Description:||This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known.|
|Source:||Human HEK293T cell line|
|Tag :||C-terminal Myc/DDK|
|Form:||1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer(25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4).
1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer.
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT).
|Molecular Mass:||103.8 kDa|
|Storage:||The lysate is shipped with dry ice. Upon receiving, store the sample at -20 centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles.
Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20 centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
|Gene Name:||DLG1 discs, large homolog 1 (Drosophila) [ Homo sapiens ]|
|Synonyms:||DLG1; discs, large homolog 1 (Drosophila); discs, large (Drosophila) homolog 1; disks large homolog 1; discs large homolog 1; dJ1061C18.1.1; DLGH1; hdlg; presynaptic protein SAP97; SAP 97; SAP97; synapse associated protein 97; synapse-associated protein 97; SAP-97; DKFZp761P0818; DKFZp781B0426;|
|Pathway:||Activation of Ca-permeable Kainate Receptor, organism-specific biosystem; Activation of Kainate Receptors upon glutamate binding, organism-specific biosystem; Axon guidance, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem; Developmental Biology, organism-specific biosystem; E-cadherin signaling in the nascent adherens junction, organism-specific biosystem; Glutamate Binding, Activation of AMPA Receptors and Synaptic Plasticity, organism-specific biosystem;|
|Function:||L27 domain binding; cytoskeletal protein binding; guanylate kinase activity; phosphatase binding; phosphoprotein phosphatase activity; potassium channel regulator activity; protein C-terminus binding; protein binding; protein kinase binding;|
Price does not include shipping and packaging costs.Request quote for final price.