Recombinant Human DLL1 protein, Fc-tagged

• Cat.No.: DLL1-548H
• Product Overview: Recombinant Human Delta-like Protein 1 is produced by our Mammalian expression system and the target gene encoding Gln18-Gly540 is expressed with a Fc tag at the C-terminus.

Specification

• Description: Delta-like protein 1 (DLL1) is a type I transmembrane protein that belongs to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain. Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. The residual membranebound portion of DLL1 can be cleave by presenilin-dependent γ-secretase, enabling the cytoplasmic domain to migrate to the nucleus. DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1. DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation. The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis. DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression.
• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from a 0.2 μm filtered solution of 20mM Hepes, 150mM NaCl, 1mM EDTA, pH 7.4.
• Molecular Mass: 90-100kDa, reducing conditions.
• Protein length: Gln18-Gly540
• Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20°C for 3 months.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
  It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: DLL1 delta like canonical Notch ligand 1 [ Homo sapiens (human) ]
• Official Symbol: DLL1
• Synonyms: Delta-like protein 1; Drosophila Delta homolog 1; Delta1; H-Delta-1; DLL1
• Gene ID: 28514
• mRNA Refseq: NM_005618.4
• Protein Refseq: NP_005609.3
• MIM: 606582
• UniProt ID: O00548