Active Recombinant Human DNMT3A/DNMT3L Complex, His/GST-tagged

• Cat.No.: DNMT3A/DNMT3L-0293H
• Product Overview: Recombinant Human DNMT3A(a.a 623-912) fused with His tag at N terminal and DNMT3L(a.a. 160-387) fused with GST tag at N terminal complex was co-expressed in a baculovirus-infected Sf9 cell expression system.

Specification

• Source: Insect cells
• Species: Human
• Tag: His/GST
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 16 mM glutathione and 20% glycerol.
• Bio-activity: ≥0.58 pmol/min/µg.
• Molecular Mass: DNMT3A: 34 kDa; DNMT3L: 52 kDa
• Purity: > 95%
• Applications: Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.
• Storage: >6 months at –80 centigrade. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: DNMT3A DNA (cytosine-5-)-methyltransferase 3 alpha [ Homo sapiens ]
• Official Symbol: DNMT3A
• Synonyms: DNMT3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA (cytosine-5)-methyltransferase 3A; DNA MTase HsaIIIA; DNA cytosine methyltransferase 3A2; DNMT3A2; M.HsaIIIA
• Gene ID: 1788
• mRNA Refseq: NM_175629
• Protein Refseq: NP_783328
• MIM: 602769
• UniProt ID: Q9Y6K1
• Chromosome Location: 2p23
• Pathway: Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Methionine degradation, organism-specific biosystem; Methionine degradation, conserved biosystem; One Carbon Metabolism, organism-specific biosystem; Validated targets of C-MYC transcriptional repression, organism-specific biosystem
• Function: DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; DNA binding; chromatin binding; metal ion binding; methyltransferase activity; protein binding; transferase activity; unmethylated CpG binding