Recombinant Human DNTT Protein, His-tagged

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Recombinant Human DNTT Protein, His-tagged

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Cat.No. : DNTT-2569H
Product Overview : Recombinant Human DNTT(162 - 509 aa) fused with His tag at N-terminal was expressed in E. coli.
Description : This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
Source : E. coli
Species : Human
Tag : His
Form : The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.). Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
Protein length : 162 - 509 aa
AA Sequence : NNCNQIFTDA FDILAENCEF RENEDSCVTF MRAASVLKSL PFTIISMKDT EGIPCLGSKV KGIIEEIIED GESSEVKAVL NDERYQSFKL FTSVFGVGLK TSEKWFRMGF RTLSKVRSDK SLKFTRMQKA GFLYYEDLVS CVTRAEAEAV SVLVKEAVWA FLPDAFVTMT GGFRRGKKMG HDVDFLITSP GSTEDEEQLL QKVMNLWEKK GLLLYYDLVE STFEKLRLPS RKVDALDHFQ KCFLIFKLPR QRVDSDQSSW QEGKTWKAIR VDLVLCPYER RAFALLGWTG SRQFERDLRR YATHERKMIL DNHALYDKTK RIFLKAESEE EIFAHLGLDY IEPWERNA
Purity : 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
Storage : Store for up to 12 months at -20 centigrade to -80 centigrade as lyophilized powder.
Storage of Reconstituted Protein:
Short-term storage: Store at 2-8 centigrade for two weeks.
Long-term storage: Aliquot and store at -20 centigrade to -80 centigrade for up to 6 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
Reconstitution : Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
Shipping : The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature (see below).
Figure :

Gene Name : DNTT deoxynucleotidyltransferase, terminal [ Homo sapiens ]
Official Symbol : DNTT
Synonyms : DNTT; deoxynucleotidyltransferase, terminal; DNA nucleotidylexotransferase; TDT; Terminal deoxynucleotidyltransferase; terminal transferase; terminal addition enzyme; terminal deoxynucleotidyltransferase; terminal deoxyribonucleotidyltransferase; nucleosidetriphosphate:DNA deoxynucleotidylexotransferase;
Gene ID : 1791
mRNA Refseq : NM_001017520
Protein Refseq : NP_001017520
MIM : 187410
UniProt ID : P04053

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