Recombinant Human DPEP1 protein, His-tagged

• Cat.No.: DPEP1-8595H
• Product Overview: Recombinant Human DPEP1 protein(P16444) (Met1-Ser385) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-385 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The recombinant human DPEP1 consists of 380 amino acids and predicts a molecular mass of 42.5 KDa. It migrates as an approximately 44-47 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DPEP1 dipeptidase 1 (renal) [ Homo sapiens ]
• Official Symbol: DPEP1
• Synonyms: MDP; RDP; MBD1
• Gene ID: 1800
• mRNA Refseq: NM_004413.3
• Protein Refseq: NP_004404.1
• MIM: 179780
• UniProt ID: P16444

Citation

Dipeptidase-1 Is an Adhesion Receptor for Neutrophil Recruitment in Lungs and Liver.

Journal: Cell    PubMed ID: 31442408    Data: 2020/5/11

Authors: Saurav Roy Choudhury, Liane Babes, Donna L Senger

Article Snippet:Agarose beads were quenched with 1M Tris, washed and resuspended in a 2X volume of PBS.Agarose beads were quenched with 1M Tris, washed and resuspended in a 2X volume of PBS.. 2 mg of C-terminal His-tagged recombinant DPEP1 (Creative BioMart, DPEP1-77H) and 30 mL agarose beads linked to LSALT, scrambled LSALT, GFE1 or GFE2 peptide were incubated in 200 ml of binding buffer (50 mM Tris pH 7.5, 150 mM NaCl, 0.05% NP-40) for 4 hours at 4 C. For the competition assay, 2 mg of unconjugated LSALT peptide was added to the recombinant DPEP1 and LSALT-agarose beads mixture.. Beads were washed three times with binding buffer and boiled with 50 mL of 2X Laemmli buffer.Beads were washed three times with binding buffer and boiled with 50 mL of 2X Laemmli buffer.