Recombinant Human DPY30 protein, His-tagged

• Cat.No.: DPY30-315H
• Product Overview: Recombinant full-length human DPY30 was expressed in E.coli cells using an N-terminal His tag.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: As a core subunit of the SET1/MLL histone methyl-transferase complexes, DPY30 modulates H3K4 methylation in vitro, and directly regulates chromosomal H3K4 trimethylation (H3K4me3) throughout the mam-malian genome. The methylation event has been shown to affect embryonic stem cells (ESCs) maintenance and differentiation. Depletion of Dpy-30 does not affect ESC self-renewal, but significantly alters the differentiation potential of ESCs, particularly along the neural lineage.
• Form: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol
• Molecular Mass: 20 kDa
• Purity: The purity of DPY30 was determined to be >95% by densitometry.
• Applications: Enzyme Assay; WB
• Stability: 1 year at -70 centigrade
• Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL

Gene Information

• Gene Name: histone methyltransferase complex regulatory subunit [ Homo sapiens (human) ]
• Official Symbol: DPY30
• Synonyms: Cps25; Saf19; HDPY-30
• Gene ID: 84661
• mRNA Refseq: NM_032574
• Protein Refseq: NP_115963
• MIM: 612032
• UniProt ID: Q9C005