Recombinant Human DROSHA protein, GST-tagged

• Cat.No.: DROSHA-3741H
• Product Overview: Recombinant Human DROSHA protein(353-550 aa), fused to GST tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: GST
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 100mM GSH.
• Protein length: 353-550 aa
• AA Sequence: MHRSPSREKKRARWEEEKDRWSDNQ SSGKDKNYTSIKEKEPEETMPDKNE EEEEELLKPVWIRCTHSENYYSSDP MDQVGDSTVVGTSRLRDLYDKFEEE LGSRQEKAKAARPPWEPPKTKLDED LESSSESECESDEDSTCSSSSDSEV FDVIAEIKRKKAHPDRLHDELWYND PGQMNDGPLCKCSAKARRTGIRHS
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: DROSHA drosha, ribonuclease type III [ Homo sapiens ]
• Official Symbol: DROSHA
• Synonyms: DROSHA; drosha, ribonuclease type III; ribonuclease type III, nuclear , RNASEN; ribonuclease 3; drosha; double stranded RNA specific endoribonuclease; ribonuclease type III; Etohi2; HSA242976; RN3; RNASE3L; p241; RNase III; protein Drosha; nuclear RNase III Drosha; putative ribonuclease III; ribonuclease III, nuclear; ribonuclease type III, nuclear; drosha, double-stranded RNA-specific endoribonuclease; putative protein p241 which interacts with transcription factor Sp1; ETOHI2; RNASEN; RANSE3L
• Gene ID: 29102
• mRNA Refseq: NM_001100412
• Protein Refseq: NP_001093882
• MIM: 608828
• UniProt ID: Q9NRR4