Recombinant Human DTD2 Protein, Myc/DDK-tagged, C13 and N15-labeled
Cat.No. : | DTD2-2792H |
Product Overview : | C14orf126 MS Standard C13 and N15-labeled recombinant protein (NP_542395) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells. |
- Specification
- Gene Information
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Description : | Deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Probably acts by rejecting L-amino acids from its binding site rather than specific recognition of D-amino acids. Catalyzes the hydrolysis of D-tyrosyl-tRNA(Tyr), has no activity on correctly charged L-tyrosyl-tRNA(Tyr). By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality. In contrast to DTD1, deacylates L-Ala mischarged on tRNA(Thr)(G4.U69) by alanine-tRNA ligase AARS. Can deacylate L-Ala due to a relaxed specificity for substrate chirality caused by the trans conformation of the Gly-Pro motif in the active site. Also hydrolyzes correctly charged, achiral, glycyl-tRNA(Gly) in vitro, although in vivo EEF1A1/EF-Tu may protect cognate achiral glycyl-tRNA(Gly) from DTD2-mediated deacetylation. |
Source : | HEK293 |
Species : | Human |
Tag : | Myc/DDK |
Molecular Mass : | 18.7 kDa |
AA Sequence : | MAEGSRIPQARALLQQCLHARLQIR PADGDVAAQWVEVQRGLVIYVCFFK GADKELLPKMVNTLLNVKLSETENG KHVSILDLPGNILIIPQATLGGRLK GRNMQYHSNSGKEEGFELYSQFVTL CEKEVAANSKCAEARVVVEHGTYGN RQVLKLDTNGPFTHLIEFTRTRPLE QKLISEEDLAANDILDYKDDDDKV |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : | Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : | 50 μg/mL as determined by BCA |
Storage Buffer : | 100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
Gene Name : | DTD2 D-aminoacyl-tRNA deacylase 2 [ Homo sapiens (human) ] |
Official Symbol : | DTD2 |
Synonyms : | DTD2; D-aminoacyl-tRNA deacylase 2; ATD; C14orf126; D-aminoacyl-tRNA deacylase 2; D-tyrosyl-tRNA deacylase 2 (putative); D-tyrosyl-tRNA(Tyr) deacylase 2; L-alanyl-tRNA deacylase; animalia-specifc tRNA deacylase; animalia-specific tRNA deacylase; probable D-tyrosyl-tRNA(Tyr) deacylase 2; putative D-tyrosyl-tRNA deacylase 2; putative D-tyrosyl-tRNA(Tyr) deacylase 2; EC 3.1.1.96 |
Gene ID : | 112487 |
mRNA Refseq : | NM_080664 |
Protein Refseq : | NP_542395 |
UniProt ID : | Q96FN9 |
Products Types
◆ Recombinant Protein | ||
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DTD2-2824H | Recombinant Human DTD2 Protein, His (Fc)-Avi-tagged | +Inquiry |
DTD2-2718H | Recombinant Human DTD2 Protein, MYC/DDK-tagged | +Inquiry |
DTD2-5011H | Recombinant Human DTD2, His-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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