Recombinant Human DTD2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: DTD2-2792H
• Product Overview: C14orf126 MS Standard C13 and N15-labeled recombinant protein (NP_542395) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: Deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Probably acts by rejecting L-amino acids from its binding site rather than specific recognition of D-amino acids. Catalyzes the hydrolysis of D-tyrosyl-tRNA(Tyr), has no activity on correctly charged L-tyrosyl-tRNA(Tyr). By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality. In contrast to DTD1, deacylates L-Ala mischarged on tRNA(Thr)(G4.U69) by alanine-tRNA ligase AARS. Can deacylate L-Ala due to a relaxed specificity for substrate chirality caused by the trans conformation of the Gly-Pro motif in the active site. Also hydrolyzes correctly charged, achiral, glycyl-tRNA(Gly) in vitro, although in vivo EEF1A1/EF-Tu may protect cognate achiral glycyl-tRNA(Gly) from DTD2-mediated deacetylation.
• Molecular Mass: 18.7 kDa
• AA Sequence: MAEGSRIPQARALLQQCLHARLQIRPADGDVAAQWVEVQRGLVIYVCFFKGADKELLPKMVNTLLNVKLSETENGKHVSILDLPGNILIIPQATLGGRLKGRNMQYHSNSGKEEGFELYSQFVTLCEKEVAANSKCAEARVVVEHGTYGNRQVLKLDTNGPFTHLIEFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: DTD2 D-aminoacyl-tRNA deacylase 2 [ Homo sapiens (human) ]
• Official Symbol: DTD2
• Synonyms: DTD2; D-aminoacyl-tRNA deacylase 2; ATD; C14orf126; D-aminoacyl-tRNA deacylase 2; D-tyrosyl-tRNA deacylase 2 (putative); D-tyrosyl-tRNA(Tyr) deacylase 2; L-alanyl-tRNA deacylase; animalia-specifc tRNA deacylase; animalia-specific tRNA deacylase; probable D-tyrosyl-tRNA(Tyr) deacylase 2; putative D-tyrosyl-tRNA deacylase 2; putative D-tyrosyl-tRNA(Tyr) deacylase 2; EC 3.1.1.96
• Gene ID: 112487
• mRNA Refseq: NM_080664
• Protein Refseq: NP_542395
• UniProt ID: Q96FN9