Active Recombinant Human DYRK1B Protein, GST-tagged

• Cat.No.: DYRK1B-2983H
• Product Overview: Human DYRK1B (NP_004705.1, 1 a.a. - 629 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.

Specification

• Description: This gene encodes a member of a family of nuclear-localized protein kinases. The encoded protein participates in the regulation of the cell cycle. Expression of this gene may be altered in tumor cells, and mutations in this gene were found to cause abdominal obesity-metabolic syndrome 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
• Source: Insect cells
• Species: Human
• Tag: GST
• Form: Liquid
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000.
  Substrate: DYRKtide-F.
  ATP: 100 μM.
• Molecular Mass: 96 kDa
• Purity: 81 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
• Publication:
  Dyrk1b promotes hepatic lipogenesis by bypassing canonical insulin signaling and directly activating mTORC2 in mice (2021)

Gene Information

• Gene Name: DYRK1B dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B [ Homo sapiens ]
• Official Symbol: DYRK1B
• Synonyms: DYRK1B; dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B; dual specificity tyrosine-phosphorylation-regulated kinase 1B; minibrain related kinase; MIRK; mirk protein kinase; minibrain-related kinase
• Gene ID: 9149
• mRNA Refseq: NM_004714
• Protein Refseq: NP_004705
• MIM: 604556
• UniProt ID: Q9Y463