Recombinant Human E4F1, His-tagged

• Cat.No.: E4F1-27377TH
• Product Overview: Recombinant fragment, corresponding to amino acids 137-588 of Human E4F1 with an N terminal His tag. Predicted MWt: 50 kDa,

Specification

• Description: The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence.
• Conjugation: HIS
• Source: E. coli
• Form: Lyophilised:Reconstitute with 87 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: LVGGGHIKEVIVAAEAELGDGEMAE APGSPRQQGLGLAGE GEQAQVKLLVNKDGRYVCALCHKTF KTGSILKAHMVTH SSRKDHECKLCGASFRTKGSLIRHH RRHTDERPYKCSK CGKSFRESGALTRHLKSLTPCTEKI RFSVSKDVVVSKEDA RAGSGAGAAGLGTATSSVTGEPIET SPVIHLVTDAKGT VIHEVHVQMQELSLGMKALAPEPPV SQELPCSSEGSRE NLLHQAMQNSGIVLERAAGEEGALE PAPAAGSSPQPLA VAAPQLPVLEVQPLETQVASEASAV PRTHPCPQCSETFPT AATLEAHKRGHTGPRPFACAQCGKA FPKAYLLKKHQEV HVRERRFRCGDCGKLYKTIAHVRGH RRVHSDERPYPCP KCGKRYKTKNAQQVHFRTHLEEKPH VCQFCSRGFREKGSLVRHVRHHTGE KPFKCYKCGRGFAEHG

Gene Information

• Gene Name: E4F1 E4F transcription factor 1 [ Homo sapiens ]
• Official Symbol: E4F1
• Synonyms: E4F1; E4F transcription factor 1; transcription factor E4F1; E4F
• Gene ID: 1877
• mRNA Refseq: NM_004424
• Protein Refseq: NP_004415
• MIM: 603022
• Uniprot ID: Q66K89
• Chromosome Location: 16p13.3
• Pathway: p53 pathway, organism-specific biosystem
• Function: DNA binding; cAMP response element binding; ligase activity; metal ion binding; protein binding