Recombinant Human EIF2B2, T7-tagged

• Cat.No.: EIF2B2-1535H
• Product Overview: Recombinant Human EIF2B2 was expressed inE.Coliusing an N-terminal T7 tag. MW=40.4 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: T7
• Description: Eukaryotic initiation factor-2B (EIF2B) is a GTP exchange protein essential for protein synthesis. It consists of α (EIF2B1; 606686), β (EIF2B2), γ (EIF2B3; 606273), δ (EIF2B4; 606687), and epsilon (EIF2B5; 603945) subunits. EIF2B activates its EIF2 (see 603907) substrate by exchanging EIF2-bound GDP for GTP.
• Sequence: 1-351.
• Purity: ~95%.
• Specific Activity: n/aH.
• Preparation: 0.1mg/0.37 ml.
• Applications: E, WB, MS.
• Buffer: 10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3.
• Storage: Store at -70?C. As with any protein, exposing EIF2B2 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• SDS-PAGE: SDS PAGE Analysis of EIF3S2 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.

Gene Information

• Gene Name: EIF2B2 eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa [ Homo sapiens ]
• Synonyms: EIF2B2; eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa; EIF2B; EIF-2Bbeta; eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa
• Gene ID: 8892
• mRNA Refseq: NM_014239
• Protein Refseq: NP_055054
• MIM: 606454
• UniProt ID: P49770
• Chromosome Location: 14q24.3
• Pathway: Gene Expression; Metabolism of proteins
• Function: protein binding; translation initiation factor activity; GTP binding; translation initiation factor activity; ATP binding