Recombinant Human ENDOG protein, His & GST-tagged
Cat.No. : | ENDOG-592H |
- Specification
- Gene Information
- Related Products
Product overview : | Recombinant Human ENDOG (Q14249) aa. (Met1~Lys294) fused with His & GST tag was produced in E. coli cells. |
Description : | The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. |
Source : | E. coli |
Species : | Human |
Tag : | His & GST |
Form : | Freeze-dried powder |
Molecular Mass : | Predicted Molecular Mass: 62.3kDa; Accurate Molecular Mass: 62kDa as determined by SDS-PAGE reducing conditions. |
Protein length : | Met1~Lys294 |
Purity : | >92% |
Characteristic : | Isoelectric point is 9.5. |
Applications : | Cell culture; Activity Assays; In vivo assays. |
Stability : | The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Storage buffer : | 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl and 5% trehalose. |
Reconstitution : | Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex |
Gene Name : | ENDOG endonuclease G [ Homo sapiens (human) ] |
Official Symbol : | ENDOG |
Synonyms : | ENDOG; endonuclease G; endonuclease G, mitochondrial; endo G; mitochondrial endonuclease G |
Gene ID : | 2021 |
mRNA Refseq : | NM_004435.2 |
Protein Refseq : | NP_004426.2 |
UniProt ID : | Q14249 |
Products Types
◆ Recombinant Protein | ||
Endog-697R | Recombinant Rat Endog Protein, His/GST-tagged | +Inquiry |
ENDOG-696H | Recombinant Human ENDOG Protein, His-tagged | +Inquiry |
ENDOG-2788M | Recombinant Mouse ENDOG Protein, His (Fc)-Avi-tagged | +Inquiry |
ENDOG-3305H | Recombinant Human ENDOG Protein, GST-tagged | +Inquiry |
ENDOG-5197M | Recombinant Mouse ENDOG Protein | +Inquiry |
◆ Lysates | ||
ENDOG-6602HCL | Recombinant Human ENDOG 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionThe structure of ENDOG includes multiple functional domains, including a hydrophobic transmembrane domain at the N-terminus, an uncoupling domain at the intermediate, and a proton pump domain at the C-terminus.
It catalyzes the electron transfer of NADH+H⁺ to the outside mitochondria via the uncoupling domain, while converting the proton electrochemical gradient of the inner mitochondrial membrane into heat energy.
ENDOG regulates mitochondrial energy metabolism and nutrient metabolism processes through uncoupling and proton pump action. For example, it can transfer electrons of NADH+H⁺ to the outside of mitochondria while converting the proton electrochemical gradient of the inner mitochondrial membrane into heat energy.
ENDOG HAS A VARIETY OF ASSOCIATIONS WITH OTHER PROTEINS. For example, it may interact with other proteins on the inner mitochondrial membrane to co-participate in mitochondrial function, and it may also interact with proteins in signal transduction pathways to participate in cell signaling.
There are differences in the expression levels of ENDOG in different tissues and cells. For example, it is expressed higher in muscle tissue and lower in brain tissue. This difference may be related to the energy requirements and metabolic characteristics of different tissues and cells.
Abnormal ENDOG levels may indicate a variety of diseases, especially tumors, neurodegenerative diseases, etc. For example, in tumors such as lung, breast, and colon cancer, expression levels of ENDOG may be abnormally elevated.
Customer Reviews (3)
Write a reviewThis protein synthesis process is efficient and reliable, reducing experimental time and cost.
ENDOG is easy to purify and manipulate, which improves the experimental efficiency.
The sensitivity and accuracy of the experiment were significantly improved by using this protein.
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