Recombinant Human ENOPH1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ENOPH1-5490H
• Product Overview: ENOPH1 MS Standard C13 and N15-labeled recombinant protein (NP_067027) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 28.9 kDa
• AA Sequence: MVVLSVPAEVTVILLDIEGTTTPIA FVKDILFPYIEENVKEYLQTHWEEE ECQQDVSLLRKQAEEDAHLDGAVPI PAASGNGVDDLQQMIQAVVDNVCWQ MSLDRKTTALKQLQGHMWRAAFTAG RMKAEFFADVVPAVRKWREAGMKVY IYSSGSVEAQKLLFGHSTEGDILEL VDGHFDTKIGHKVESESYRKIADSI GCSTNNILFLTDVTREASAAEEADV HVAVVVRPGNAGLTDDEKTYYSLIT SFSELYLPSSTTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ENOPH1 enolase-phosphatase 1 [ Homo sapiens (human) ]
• Official Symbol: ENOPH1
• Synonyms: ENOPH1; enolase-phosphatase 1; enolase-phosphatase E1; acireductone synthase; E1; Enolase phosphatase E1; MASA; MASA homolog; 2,3-diketo-5-methylthio-1-phosphopentane phosphatase; MST145; FLJ12594; DKFZp586M0524
• Gene ID: 58478
• mRNA Refseq: NM_021204
• Protein Refseq: NP_067027
• UniProt ID: Q9UHY7