Recombinant Human EPHB4 Protein, His-tagged

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Recombinant Human EPHB4 Protein, His-tagged

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Cat.No. : EPHB4-003H
Product Overview : Recombinant human EPHB4 protein(16-539aa), fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description : EPHB4, also known ephrin type-B receptor 4, is a member of the Eph receptor tyrosine kinase family. It binds transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. This protein plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. And the signaling of this protein controls cellular repulsion and segregation form EFNB2-expressing cells. And it also plays a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis.
Source : Insect cell
Species : Human
Tag : His
Form : Liquid
Molecular Mass : 58.1 kDa (532aa)
AA Sequence : LEETLLNTKL ETADLKWVTF PQVDGQWEEL SGLDEEQHSV RTYEVCDVQR APGQAHWLRT GWVPRRGAVH VYATLRFTML ECLSLPRAGR SCKETFTVFY YESDADTATA LTPAWMENPY IKVDTVAAEH LTRKRPGAEA TGKVNVKTLR LGPLSKAGFY LAFQDQGACM ALLSLHLFYK KCAQLTVNLT RFPETVPREL VVPVAGSCVV DAVPAPGPSP SLYCREDGQW AEQPVTGCSC APGFEAAEGN TKCRACAQGT FKPLSGEGSC QPCPANSHSN TIGSAVCQCR VGYFRARTDP RGAPCTTPPS APRSVVSRLN GSSLHLEWSA PLESGGREDL TYALRCRECR PGGSCAPCGG DLTFDPGPRD LVEPWVVVRG LRPDFTYTFE VTALNGVSSL ATGPVPFEPV NVTTDREVPP AVSDIRVTRS SPSSLSLAWA VPRAPSGAVL DYEVKYHEKG AEGPSSVRFL KTSENRAELR GLKRGASYLV QVRARSEAGY GPFGQEHHSQ TQLDESEGWR EQLAVEHHHH HH
N-terminal Sequence Analysis : Leu 16
Endotoxin : < 1.0 EU per 1μg of protein (determined by LAL method)
Purity : > 90% by SDS-PAGE
Notes : For research use only. This product is not intended or approved for human, diagnostics or veterin.
Storage : Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 centigrade.
Avoid repeated freezing and thawing cycles.
Concentration : 0.25 mg/mL (determined by Absorbance at 280nm)
Storage Buffer : In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
SDS-PAGE :

15% SDS-PAGE (3μg)
50-70 kDa (SDS-PAGE under reducing conditions.)

Gene Name : EPHB4 EPH receptor B4 [ Homo sapiens (human) ]
Official Symbol : EPHB4
Synonyms : EPHB4; HFASD; HTK; MYK1; TYRO11; Ephrin type-B receptor 4; ephrin type-B receptor 4; ephrin receptor EphB4; hepatoma transmembrane kinase; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; tyrosine-protein kinase TYRO11; tyrosine-protein kinase receptor HTK; EC 2.7.10.1
Gene ID : 2050
mRNA Refseq : NM_004444
Protein Refseq : NP_004435
MIM : 600011
UniProt ID : P54760

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