Recombinant Human EPHB4 protein(Met1-Ala539)

Cat.No. : EPHB4-697H
Product Overview : Recombinant Human EphB4 (NP_004435.3) (Met 1-Ala 539) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified in HEK293.
Availability July 25, 2024
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Source : HEK293
Species : Human
Tag : Non
Protein length : Met1-Ala539
Form : Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized human EphB4 at 2 μg/ml (100 μl/well) can bind human EphrinB2 with a linear range of 1-125 ng/ml.
Molecular Mass : The recombinant human EphB4 is a monomeric protein consisting of 530 amino acids and has a calculated molecular mass of 57.7 kDa. As a result of glycosylation, rhEphB4 migrates as an approximately 65-70 kDa protein in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 95 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : EPHB4 EPH receptor B4 [ Homo sapiens ]
Official Symbol : EPHB4
Synonyms : EPHB4; EPH receptor B4; EphB4 , HTK; ephrin type-B receptor 4; Tyro11; ephrin receptor EphB4; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; hepatoma transmembrane kinase; tyrosine-protein kinase TYRO11; tyrosine-protein kinase receptor HTK; HTK; MYK1; TYRO11;
Gene ID : 2050
mRNA Refseq : NM_004444
Protein Refseq : NP_004435
MIM : 600011
UniProt ID : P54760

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