Recombinant Human EPHX1 Protein, His-tagged

• Cat.No.: EPHX1-22H
• Product Overview: Recombinant human EPHX1 protein with His tag, was expressed in insect cell.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: EPHX1, also known as epoxide hydrolase 1, is an important bioconversion enzyme that converts epoxides from degradation of aromatics into trans-dihydrodiols that can be conjugated and excreted in the body. The epoxide hydrolase acts both in the activation and decryption of the epoxide. This enzyme plays a role in regulating the extensive oxidation state of heterologous and lipid-derived substrates. Mutations in this gene cause preeclampsia, lack of epoxidase, or increased epoxide hydrolase activity. Recombinant human EPHX1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
• Form: Liquid
• Molecular Mass: 51.5kDa (442aa) 50-70kDa (SDS-PAGE under reducing conditions)
• AA Sequence: MRDKEETLPLEDGWWGPGTRSAAREDDSIRPFKVETSDEEIHDLHQRIDKFRFTPPLEDSCFHYGFNSNYLKKVISYWRNEFDWKKQVEILNRYPHFKTKIEGLDIHFIHVKPPQLPAGHTPKPLLMVHGWPGSFYEFYKIIPLLTDPKNHGLSDEHVFEVICPSIPGYGFSEASSKKGFNSVATARIFYKLMLRLGFQEFYIQGGDWGSLICTNMAQLVPSHVKGLHLNMALVLSNFSTLTLLLGQRFGRFLGLTERDVELLYPVKEKVFYSLMRESGYMHIQCTKPDTVGSALNDSPVGLAAYILEKFSTWTNTEFRYLEDGGLERKFSLDDLLTNVMLYWTTGTIISSQRFYKENLGQGWMTQKHERMKVYVPTGFSAFPFELLHTPEKWVRFKYPKLISYSYMVRGGHFAAFEEPELLAQDIRKFLSVLERQHHHHHH
• N-terminal Sequence Analysis: Met
• Endotoxin: < 1.0 EU per 1ug of protein (determined by LAL method)
• Purity: > 85% by SDS - PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: 20mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 1mM DTT, 0.1M NaCl
• Warning: For research use only. This product is not intended or approved for human, diagnostics or veterinary use

Gene Information

• Gene Name: EPHX1 epoxide hydrolase 1 [ Homo sapiens (human) ]
• Official Symbol: EPHX1
• Synonyms: EPHX1; EPHX; EPOX; HYL1; MEH; epoxide hydrolase 1; epoxide hydrolase 1; epoxide hydratase; epoxide hydrolase 1 microsomal; epoxide hydrolase 1, microsomal (xenobiotic); EC 3.3.2.9
• Gene ID: 2052
• mRNA Refseq: NM_000120
• Protein Refseq: NP_000111
• MIM: 132810
• UniProt ID: P07099