Recombinant Human EPHX1 Protein, His-tagged

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Recombinant Human EPHX1 Protein, His-tagged

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Cat.No. : EPHX1-22H
Product Overview : Recombinant human EPHX1 protein with His tag, was expressed in insect cell.
Description : EPHX1, also known as epoxide hydrolase 1, is an important bioconversion enzyme that converts epoxides from degradation of aromatics into trans-dihydrodiols that can be conjugated and excreted in the body. The epoxide hydrolase acts both in the activation and decryption of the epoxide. This enzyme plays a role in regulating the extensive oxidation state of heterologous and lipid-derived substrates. Mutations in this gene cause preeclampsia, lack of epoxidase, or increased epoxide hydrolase activity. Recombinant human EPHX1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Source : Insect cell
Species : Human
Tag : His
Form : Liquid
Molecular Mass : 51.5kDa (442aa) 50-70kDa (SDS-PAGE under reducing conditions)
AA Sequence : MRDKEETLPLEDGWW GPGTRSAAREDDSIR PFKVETSDEEIHDLH QRIDKFRFTPPLEDS CFHYGFNSNYLKKVI SYWRNEFDWKKQVEI LNRYPHFKTKIEGLD IHFIHVKPPQLPAGH TPKPLLMVHGWPGSF YEFYKIIPLLTDPKN HGLSDEHVFEVICPS IPGYGFSEASSKKGF NSVATARIFYKLMLR LGFQEFYIQGGDWGS LICTNMAQLVPSHVK GLHLNMALVLSNFST LTLLLGQRFGRFLGL TERDVELLYPVKEKV FYSLMRESGYMHIQC TKPDTVGSALNDSPV GLAAYILEKFSTWTN TEFRYLEDGGLERKF SLDDLLTNVMLYWTT GTIISSQRFYKENLG QGWMTQKHERMKVYV PTGFSAFPFELLHTP EKWVRFKYPKLISYS YMVRGGHFAAFEEPE LLAQDIRKFLSVLER QHHHHHH
N-terminal Sequence Analysis : Met
Endotoxin : < 1.0 EU per 1ug of protein (determined by LAL method)
Purity : > 85% by SDS - PAGE
Storage : Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 0.25mg/mL (determined by Absorbance at 280nm)
Storage Buffer : 20mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 1mM DTT, 0.1M NaCl
Warning : For research use only. This product is not intended or approved for human, diagnostics or veterinary use
Figure :

Gene Name : EPHX1 epoxide hydrolase 1 [ Homo sapiens (human) ]
Official Symbol : EPHX1
Synonyms : EPHX1; EPHX; EPOX; HYL1; MEH; epoxide hydrolase 1; epoxide hydrolase 1; epoxide hydratase; epoxide hydrolase 1 microsomal; epoxide hydrolase 1, microsomal (xenobiotic); EC 3.3.2.9
Gene ID : 2052
mRNA Refseq : NM_000120
Protein Refseq : NP_000111
MIM : 132810
UniProt ID : P07099

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