| Description : |
EPHX1, also known as epoxide hydrolase 1, is an important bioconversion enzyme that converts epoxides from degradation of aromatics into trans-dihydrodiols that can be conjugated and excreted in the body. The epoxide hydrolase acts both in the activation and decryption of the epoxide. This enzyme plays a role in regulating the extensive oxidation state of heterologous and lipid-derived substrates. Mutations in this gene cause preeclampsia, lack of epoxidase, or increased epoxide hydrolase activity. Recombinant human EPHX1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Source : |
Insect cell |
| Species : |
Human |
| Tag : |
His |
| Form : |
Liquid |
| Molecular Mass : |
51.5kDa (442aa) 50-70kDa (SDS-PAGE under reducing conditions) |
| AA Sequence : |
MRDKEETLPLEDGWWGPGTRSAARE DDSIRPFKVETSDEEIHDLHQRIDK FRFTPPLEDSCFHYGFNSNYLKKVI SYWRNEFDWKKQVEILNRYPHFKTK IEGLDIHFIHVKPPQLPAGHTPKPL LMVHGWPGSFYEFYKIIPLLTDPKN HGLSDEHVFEVICPSIPGYGFSEAS SKKGFNSVATARIFYKLMLRLGFQE FYIQGGDWGSLICTNMAQLVPSHVK GLHLNMALVLSNFSTLTLLLGQRFG RFLGLTERDVELLYPVKEKVFYSLM RESGYMHIQCTKPDTVGSALNDSPV GLAAYILEKFSTWTNTEFRYLEDGG LERKFSLDDLLTNVMLYWTTGTIIS SQRFYKENLGQGWMTQKHERMKVYV PTGFSAFPFELLHTPEKWVRFKYPK LISYSYMVRGGHFAAFEEPELLAQD IRKFLSVLERQHHHHHH |
| N-terminal Sequence Analysis : |
Met |
| Endotoxin : |
< 1.0 EU per 1ug of protein (determined by LAL method) |
| Purity : |
> 85% by SDS - PAGE |
| Storage : |
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70centigrade. Avoid repeated freezing and thawing cycles. |
| Concentration : |
0.25mg/mL (determined by Absorbance at 280nm) |
| Storage Buffer : |
20mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 1mM DTT, 0.1M NaCl |
| Warning : |
For research use only. This product is not intended or approved for human, diagnostics or veterinary use |
