Recombinant Human EPHX1 Protein, His-tagged

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Recombinant Human EPHX1 Protein, His-tagged

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Cat.No. : EPHX1-22H
Product Overview : Recombinant human EPHX1 protein with His tag, was expressed in insect cell.
Description : EPHX1, also known as epoxide hydrolase 1, is an important bioconversion enzyme that converts epoxides from degradation of aromatics into trans-dihydrodiols that can be conjugated and excreted in the body. The epoxide hydrolase acts both in the activation and decryption of the epoxide. This enzyme plays a role in regulating the extensive oxidation state of heterologous and lipid-derived substrates. Mutations in this gene cause preeclampsia, lack of epoxidase, or increased epoxide hydrolase activity. Recombinant human EPHX1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Source : Insect cell
Species : Human
Tag : His
Form : Liquid
Molecular Mass : 51.5kDa (442aa) 50-70kDa (SDS-PAGE under reducing conditions)
AA Sequence : MRDKEETLPL EDGWWGPGTR SAAREDDSIR PFKVETSDEE IHDLHQRIDK FRFTPPLEDS CFHYGFNSNY LKKVISYWRN EFDWKKQVEI LNRYPHFKTK IEGLDIHFIH VKPPQLPAGH TPKPLLMVHG WPGSFYEFYK IIPLLTDPKN HGLSDEHVFE VICPSIPGYG FSEASSKKGF NSVATARIFY KLMLRLGFQE FYIQGGDWGS LICTNMAQLV PSHVKGLHLN MALVLSNFST LTLLLGQRFG RFLGLTERDV ELLYPVKEKV FYSLMRESGY MHIQCTKPDT VGSALNDSPV GLAAYILEKF STWTNTEFRY LEDGGLERKF SLDDLLTNVM LYWTTGTIIS SQRFYKENLG QGWMTQKHER MKVYVPTGFS AFPFELLHTP EKWVRFKYPK LISYSYMVRG GHFAAFEEPE LLAQDIRKFL SVLERQHHHH HH
N-terminal Sequence Analysis : Met
Endotoxin : < 1.0 EU per 1ug of protein (determined by LAL method)
Purity : > 85% by SDS - PAGE
Storage : Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 0.25mg/mL (determined by Absorbance at 280nm)
Storage Buffer : 20mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 1mM DTT, 0.1M NaCl
Warning : For research use only. This product is not intended or approved for human, diagnostics or veterinary use
Figure :

Gene Name : EPHX1 epoxide hydrolase 1 [ Homo sapiens (human) ]
Official Symbol : EPHX1
Synonyms : EPHX1; EPHX; EPOX; HYL1; MEH; epoxide hydrolase 1; epoxide hydrolase 1; epoxide hydratase; epoxide hydrolase 1 microsomal; epoxide hydrolase 1, microsomal (xenobiotic); EC 3.3.2.9
Gene ID : 2052
mRNA Refseq : NM_000120
Protein Refseq : NP_000111
MIM : 132810
UniProt ID : P07099
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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