Active Recombinant Human EPO protein, hFc-tagged

• Cat.No.: EPO-200H
• Product Overview: Recombinant Human EPO protein(NP_000790.2)(Met1-Arg193), fused with hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: hFc
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 2.0-8.0 ng/mL.2.Immobilized Recombinant Human EPO Receptor / EPOR Protein (ECD, His Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Human Erythropoietin / EPO Protein (Fc Tag), the EC50 is 5-15 ng/mL.
• Molecular Mass: The recombinant human EPO consists 404 amino acids and predicts a molecular mass of 45.1 kDa.
• Proteinlength: Met1-Arg193
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPO erythropoietin [ Homo sapiens ]
• Official Symbol: EPO
• Synonyms: EPO; erythropoietin; EP; epoetin; MVCD2; MGC138142
• Gene ID: 2056
• mRNA Refseq: NM_000799
• Protein Refseq: NP_000790
• MIM: 133170
• UniProt ID: P01588