Active Recombinant Human EPOR & CD131 Protein, Fc-tagged

• Cat.No.: EPOR & CD131-1241H
• Product Overview: Recombinant extracellular domain (Met 1-Pro 250) of human EPOR (NP_000112.1) linked with the extracellular domain (Trp 17-Trp 443) of human CD131 (NP_000386.1) by a peptide linker was fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-250;17-443 a.a.
• Predicted N Terminal: Ala 25
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its ability to bind rat EPO-His in a functional ELISA. 2. Measured by its ability to bind mouse EPO-His in a functional ELISA.
• Molecular Mass: The recombinant human EPOR&CD131/Fc chimera is a disulfide-linked homodimer of EPOR&CD131-Fc heterodimer. The reduced EPOR& CD131-Fc comprises 906 amino acids and has a calculated molecular mass of 101 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 125 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: EPOR erythropoietin receptor [ Homo sapiens ]
• Official Symbol: EPOR
• Synonyms: EPOR; erythropoietin receptor; EPO-R; MGC138358
• Gene ID: 2057
• mRNA Refseq: NM_000121
• Protein Refseq: NP_000112
• MIM: 133171
• UniProt ID: P19235
• Chromosome Location: 19p13.3-p13.2
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; EPO Receptor Signaling, organism-specific biosystem; EPO signaling pathway, organism-specific biosystem; Hematopoietic cell l
• Function: erythropoietin receptor activity; identical protein binding; protein binding; receptor activity