"EPOR/CD131" Related Products

Recombinant Human EPOR & CD131 Protein, Fc-tagged

Cat.No.: EPOR & CD131-1241H
Product Overview: Recombinant extracellular domain (Met 1-Pro 250) of human EPOR (NP_000112.1) linked with the extracellular domain (Trp 17-Trp 443) of human CD131 (NP_000386.1) by a peptide linker was fused with the Fc region of human IgG1 at the C-terminus.
Source: HEK293
Species: Human
Tag: Fc
Predicted N Terminal: Ala 25
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: 1. Measured by its ability to bind rat EPO-His in a functional ELISA. 2. Measured by its ability to bind mouse EPO-His in a functional ELISA.
Molecular Mass: The recombinant human EPOR&CD131/Fc chimera is a disulfide-linked homodimer of EPOR&CD131-Fc heterodimer. The reduced EPOR& CD131-Fc comprises 906 amino acids and has a calculated molecular mass of 101 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 125 kDa in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: EPOR erythropoietin receptor [ Homo sapiens ]
Official Symbol: EPOR
Synonyms: EPOR; erythropoietin receptor; EPO-R; MGC138358;
Gene ID: 2057
mRNA Refseq: NM_000121
Protein Refseq: NP_000112
MIM: 133171
UniProt ID: P19235
Chromosome Location: 19p13.3-p13.2
Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; EPO Receptor Signaling, organism-specific biosystem; EPO signaling pathway, organism-specific biosystem; Hematopoietic cell l
Function: erythropoietin receptor activity; identical protein binding; protein binding; receptor activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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