Recombinant Human ESD, GST-tagged

• Cat.No.: ESD-12554H
• Product Overview: Recombinant Human ESD protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 1-282a.a.
• Description: This gene encodes a serine hydrolase that belongs to the esterase D family. The encoded enzyme is active toward numerous substrates including O-acetylated sialic acids, and it may be involved in the recycling of sialic acids. This gene is used as a genetic marker for retinoblastoma and Wilsons disease.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: ESD esterase D [ Homo sapiens ]
• Official Symbol: ESD
• Synonyms: ESD; esterase D; esterase D/formylglutathione hydrolase; S-formylglutathione hydrolase; S formylglutathione hydrolase; esterase 10; methylumbelliferyl-acetate deacetylase; FGH; FLJ11763
• Gene ID: 2098
• mRNA Refseq: NM_001984
• Protein Refseq: NP_001975
• MIM: 133280
• UniProt ID: P10768
• Chromosome Location: 13q14.1-q14.2
• Pathway: formaldehyde oxidation II (glutathione-dependent), organism-specific biosystem
• Function: S-formylglutathione hydrolase activity; carboxylesterase activity; hydrolase activity, acting on ester bonds; methylumbelliferyl-acetate deacetylase activity

Citation

A new activator of esterase D decreases blood cholesterol level through ESD/JAB1/ABCA1 pathway.

Journal: Journal of cellular physiology    PubMed ID: 33615471    Data: 2021/10/4

Authors: Xinpeng Chen, Le Su, Junying Miao

Article Snippet: Recombinant human ESD proteins (ESD‐6541HCL) were synthesized by Creative BioMart. . The polypeptides (HHHHHHDGET*MIIMDSFALP) of JAB1 carried an acetyl group at Thr89 (86‐99aa) were synthesized with a His‐tag by the Sangon Biotech.The polypeptides (HHHHHHDGET*MIIMDSFALP) of JAB1 carried an acetyl group at Thr89 (86‐99aa) were synthesized with a His‐tag by the Sangon Biotech.