Recombinant Human EXOSC10 protein, His-tagged

• Cat.No.: EXOSC10-5391H
• Product Overview: Recombinant Human EXOSC10 fused with His tag was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: EXOSC10 is a nucleolar protein and represents one crucial component of the so-called "PM/Scl complex" or "nuclear exosome", which is an RNA-processing complex consisting of a number of exoribonucleases (not to be confused with certain multivesicular bodies which are shed by cultured cells into the culture fluid and are also called exosomes).
• Form: EXOSC10 is supplied at a concentration of 0.8mg/ml in 20mM HEPES, pH-7.5, 100mM NaCl, 0.01mM EDTA, 0.02% SDS.
• Molecular Mass: 120 kDa
• Purity: Greater than 70% as determined by SDS-PAGE.
• Applications: Western-Blot with monoclonal anti-hexa-His-tag antibody & polymyositis patient sera.
• Notes: Coating concentration: 0.4-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
• Storage: Store at 4 centigrade if entire vial will be used within 2-4 weeks.Store, frozen at -20 centigrade for longer periods of time.Avoid multiple freeze-thaw cycles.

Gene Information

• Gene Name: EXOSC10 exosome component 10 [ Homo sapiens ]
• Official Symbol: EXOSC10
• Synonyms: EXOSC10; exosome component 10; PMSCL2, polymyositis/scleroderma autoantigen 2, 100kDa; p2; p3; p4; PM Scl; PM/Scl 100; polymyositis/scleroderma autoantigen 2 (100kD); RRP6; Rrp6p; autoantigen PM-SCL; autoantigen PM/Scl 2; polymyositis/scleroderma autoantigen 100 kDa; polymyositis/scleroderma autoantigen 2, 100kDa; P100 polymyositis-scleroderma overlap syndrome-associated autoantigen; PMSCL; PM-Scl; PMSCL2; PM/Scl-100
• Gene ID: 5394
• mRNA Refseq: NM_001001998
• Protein Refseq: NP_001001998
• MIM: 605960
• UniProt ID: Q01780
• Chromosome Location: 1p36.22
• Pathway: RNA degradation, organism-specific biosystem; RNA degradation, conserved biosystem
• Function: 3-5 exonuclease activity; RNA binding; exoribonuclease activity; hydrolase activity; nucleotide binding; protein binding