Recombinant Human FASN protein, MYC/DDK-tagged

• Cat.No.: FASN-138H
• Product Overview: Recombinant Human FASN fused with MYC/DDK tag at C-terminal was expressed in HEK293.

Specification

• Description: The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
• Source: HEK293
• Species: Human
• Tag: MYC/DDK
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 273.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: FASN fatty acid synthase [ Homo sapiens ]
• Official Symbol: FASN
• Synonyms: FASN; fatty acid synthase; FAS; SDR27X1; short chain dehydrogenase/reductase family 27X; member 1; short chain dehydrogenase/reductase family 27X, member 1; OA-519; MGC14367; MGC15706
• Gene ID: 2194
• mRNA Refseq: NM_004104
• Protein Refseq: NP_004095
• MIM: 600212
• UniProt ID: P49327
• Chromosome Location: 17q25
• Pathway: ChREBP activates metabolic gene expression, organism-specific biosystem; Fatty Acid Biosynthesis, organism-specific biosystem; Fatty Acyl-CoA Biosynthesis, organism-specific biosystem; Fatty acid biosynthesis, organism-specific biosystem; Fatty acid biosynthesis, conserved biosystem; Fatty acid biosynthesis, elongation, organism-specific biosystem; Fatty acid biosynthesis, elongation, conserved biosystem
• Function: 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity; 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity; 3-oxoacyl-[acyl-carrier-protein] synthase activity; [acyl-carrier-protein] S-acetyltransferase activity; [acyl-carrier-protein] S-malonyltransferase activity; acyl carrier activity; cofactor binding; enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity; fatty acid synthase activity; hydrolase activity, acting on ester bonds; lyase activity; myristoyl-[acyl-carrier-protein] hydrolase activity; nucleotide binding; oleoyl-[acyl-carrier-protein] hydrolase activity; oxidoreductase activity; palmitoyl-[acyl-carrier-protein] hydrolase activity; phosphopantetheine binding; protein binding; transferase activity; zinc ion binding