| Species : |
Human |
| Source : |
CHO |
| Tag : |
Avi&His |
| Protein Length : |
Biotin |
| Conjugation/Label : |
46-217 aa |
| Description : |
CD32B (FCGR2B) and CD32C (FCGR2C) are 40 kDa, type I transmembrane proteins belonging to the Ig superfamily of low-affinity IgG Fc receptors. CD32B contains an immunoreceptor tyrosine-based inhibition motif (ITIM) in its cytoplasmic tail, while CD32C has an immunoreceptor tyrosine-based activation motif (ITAM). Both receptors can bind monomeric IgG and IgG complexes, and are expressed on B cells, monocytes, macrophages, granulocytes, platelets, and mast cells. CD32B primarily acts as a negative regulator, inhibiting immune cell activation, proliferation, endocytosis, phagocytosis, and degranulation. In contrast, CD32C has an activating function, contributing to immune responses. Together, they play crucial roles in regulating immune cell functions and maintaining immune homeostasis. CD32B and CD32C share almost identical extracellular domain. The aa sequence of Fc gamma RIIB and Fc gamma RIIC are the same for the region expressed (Ala46-Pro217) |
| Form : |
Liquid, 1×PBS buffer, pH7.4, 0.22 μm filtered |
| Molecular Mass : |
The protein has a predicted molecular weight of 23.5 kDa. Under DTT-reducing conditions, it migrates at approximately 35 kDa on SDS-PAGE. |
| Endotoxin : |
Less than 0.1 EU/μg protein as determined by the LAL method |
| Purity : |
>95% based on SDS-PAGE under reducing condition |
| Applications : |
ELISA, BLI |
| Storage : |
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4 centigrade for up to 2 weeks, or at -20 centigrade or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 μm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70 centigrade to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability. |
| Concentration : |
25 μg size is bottled at 0.2 mg/mL concentration. 100 μg size is supplied at a lot-specific concentration. |
