Active Recombinant Human FCGR3B protein(NA2), His-Avi-tagged, Biotinylated

• Cat.No.: FCGR3B-431H
• Product Overview: Biotinylated Recombinant Human FCGR3B protein(NA2)(O75015-1)(Gly17-Ser200) is expressed from HEK293 with His tag and Avi tag at the C-Terminus. The CD16b NA1 differ with the CD16b NA2 in AA36, 65, 82, and 106. The CD16b NA1form carries R36, N65, D82, and V106, while the CD16b NA2 form carries S36, S65, N82, and I106.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 17-200 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Biotinylated Human Fc gamma RIIIB (NA2), His Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 3.50 μM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 23.7 kDa. Due to glycosylation, the protein migrates to 47-53 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: FCGR3B Fc fragment of IgG, low affinity IIIb, receptor (CD16b) [ Homo sapiens ]
• Official Symbol: FCGR3B
• Synonyms: FCGR3B; Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc fragment of IgG, low affinity IIIb, receptor for (CD16) , FCG3, FCGR3; low affinity immunoglobulin gamma Fc region receptor III-B; CD16; CD16b; fc-gamma RIII; fc-gamma RIIIb; fc-gamma RIII-beta; igG Fc receptor III-1; Fc-gamma receptor IIIb (CD 16); Fc fragment of IgG, low affinity IIIb, receptor for (CD16); FCG3; FCGR3; FCR-10; FCRIII; FCRIIIb
• Gene ID: 2215
• mRNA Refseq: NM_000570
• Protein Refseq: NP_000561
• MIM: 610665
• UniProt ID: O75015